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KMID : 0603820150210020103
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Journal of Experimental & Biomedical Science
2015 Volume.21 No. 2 p.103 ~ p.114
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Inhibitory Effects of Rice Bran Water Extract Fermented Lactobacillus plantarum due to cAMP-dependent Phosphorylation of VASP (Ser157) on human Platelet Aggregation
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Kim Hyun-Hong
Lee Dong-Ha
Hong Jeong-Hwa
Pajaree Ingkasupart
Nam Gi-Suk
Ok Woo-Jeong
Kim Min-Ji
Yu Young-Bin
Kang Hyo-Chan
Park Hwa-Jin
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Abstract
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In this study, we investigated the effect of rice bran water extract fermented with Lactobacillus plantarum KCCM-12116 (RBLp) on ADP (20 μM)-, collagen (10 μg/mL)-, and thrombin (0.2 U/mL)-stimulated platelet aggregation. RBLp dose-dependently inhibited ADP-, collagen-, and thrombin-induced platelet aggregation, with IC50 values of 501.1, 637.2, and > 2,000 μg/mL, respectively. The platelet aggregation induced by ADP plus RBLp (750 μg/mL) was increased by the adenylate cyclase inhibitor, SQ22536, and the cAMP-dependent protein kinase (A-kinase) inhibitor, Rp-8-Br-cAMPS. Treatment with RBLp increased the phosphorylation of VASP (Ser157), an A-kinase substrate, which was also inhibited by SQ22536 and Rp-8-Br-cAMPS. It is thought that the RBLp-induced increases in cAMP contributed to the phosphorylation of VASP (Ser157), which in turn resulted in an inhibition of ADP-induced platelet aggregation, thereby indicating that RBLp has an antiplatelet effect via cAMP-dependent phosphorylation of VASP (Ser157). Thus, RBLp may have therapeutic potential for the treatment (or prevention) of platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.
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KEYWORD
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cAMP, cAMP-dependent protein kinase, Platelet aggregation, Rice bran fermentation, Vasodilatorstimulated phosphoprotein-Ser157 phosphorylation
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